Fig. 3

ASIC2 promotes proliferation and colony formation of CRC cells. a Proliferation of SW480 and HCT116 cells transfected with ASIC2 or vector were analyzed by CCK8 assay. The OD value was assessed at 1, 2, 3, 4, and 5 days, respectively. Each sample was tested in triplicates. b After 14 days of culture, the cells were stained with crystal violet and colonies containing more than 50 cells were counted. c Proliferation curve of SW620 (left panel) and HT29 (right panel) cells with or without ASIC2 knockdown. d Colony formation of SW620 (left panel) and HT29 (right panel) cells with or without ASIC2 knockdown. e SW480 cells transfected with ASIC2 or vector were injected subcutaneously into BALB/c nude mice. Mice were euthanized 3 weeks after transplantation, and tumors were excised and weighted. The tumors originated from SW480 cells transfected with ASIC2 were larger than that of control. Scale bar, 1 cm. f ASIC2 knockdown in SW620 cells inhibited tumor growth in vivo. Scale bar, 1 cm; *p < 0.05, **p < 0.01