Fig. 4

Ibrutinib induces G1 phase arrest through regulating the expression of cell cycle-related proteins. (a-d) U251 and U87 cells were incubated with ibrutinib for 12 h. The cell cycle profile was evaluated using flow cytometry. The data from three independent experiments are expressed as the mean ± SEM. (e and f) The effects of ibrutinib on the expression levels of cell cycle-related protein. U87 and U251 cells were treated with ibrutinib for 12 h. The protein extracts were examined using Western blot analysis with the indicated antibodies