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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Resveratrol inhibits Extranodal NK/T cell lymphoma through activation of DNA damage response pathway

Fig. 5

RSV actives DNA damage response pathway in NKTCL cells. a Cells were treated with 25 μM RSV for different time, the protein level of pATM (S1981) and γH2A.X (S139) in SNT-8, SNK-10 and SNT-16 cells was detected with western blot analysis. b The expression and foci of pATM (S1981) in SNT-8 cells after treated with 25 μM RSV for 6 h was determined by immunofluorescence. Cell nuclei were stained by DAPI. Images were acquired using confocal laser-scanning microscope (Scale bar, 20 μm). c Cells were incubated with RSV (25 μM), ATM inhibitor KU55933 (10 μM), or the combination of RSV and KU55933 for 6 h. The protein levels of pATM (S1981), ATM, γH2A.X (S139), pChk2 (T68), pp53 (S15), p53 were monitored. RSV enhanced the expression of pATM, and its down-stream molecules pChk2, γH2A.X and pp53 as well. While KU55933 inhibited the phosphorylation of ATM, p53, Chk2, but not H2A.X. When combined with RSV, KU55933 can diminish the activation level of pATM, pChk2 and pp53 induced by RSV, but not γH2A.X. d Cells were incubated with DMSO, RSV (25 μM), ATM inhibitor KU55933 (10 μM), or the combination of RSV and KU55933 for 48 h. The apoptosis was analyzed by Flow cytometry in each group. RSV alone increased cell apoptosis, while KU55933 alone showed no effect. When combining RSV and KU55933 together, the increased apoptosis induced by RSV was reversed

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