Table 1 Primers sequences
Primers | Sequences |
|---|---|
Kindlin-2-F | 5′-GAGGATCCCCGGGTACCGGTCGCCACCATGGCTCTGGACGGGATAAGGATG-3’ |
Kindlin-2-R | 5′-TCCTTGTAGTCCATACCCACCCAACCACTGGTAAGTTTG-3’ |
Kindlin-2-sh1 | 5′-GGACAGTTCTTACAACTTA-3’ |
Kindlin-2-sh2 | 5′-GAAGAACTTTCTCTCTTAA-3’ |
Kindlin-2-sh3 | 5′-ACTGATATAACTCCTGAAT-3’ |
shcontrol | 5′-TTCTCCGAACGTGTCACGT-3’ |
Kindlin-2-sgRNA1-F | 5′-CACCGCAGATGGCTGCTACGCGGAC-3’ |
Kindlin-2-sgRNA1-R | 5′-AAACGTCCGCGTAGCAGCCATCTGC-3’ |
Kindlin-2-sgRNA2-F | 5′-CACCGCGCGGTTCAGGTCCGTCACA-3’ |
Kindlin-2-sgRNA2-R | 5′-AAACTGTGACGGACCTGAACCGCGC-3’ |
Kindlin-2-sgRNA3-F | 5′-CACCGTCAGGGTGACATCGCGGTTC-3’ |
Kindlin-2-sgRNA3-R | 5′-AAACGAACCGCGATGTCACCCTGAC-3’ |
Kindlin2-genomic-F | 5′-CTGCGAATTCGGTGGGAT-3’ |
Kindlin2-genomic-R | 5′-TTCTCAAATGGGCCCCTC-3’ |
Axin2-F | 5′-AGTGTGAAGGCCAATGGCAG-3’ |
Axin2-R | 5′-GTATCGTCTGCGGGTCTTCC-3’ |
GAPDH-F | 5′-GCCGCATCTTCTTTTGCGTC-3’ |
GAPDH-R | 5′-TACGACCAAATCCGTTGACTCC-3’ |