Fig. 5

LASP1 is a direct target of miR-203a-3p in NPC cells. Wt or Mt. of the LASP1 mRNA 3′-UTR sequences targeted by miR-203a-3p a. Quantification of LASP1 mRNA levels by quantitative RT-PCR b and LASP1 protein expression by western blotting c after transfection with miR-203a-3p mimic or miR-Ctrl. Relative luciferase activity of CNE-2 and SUNE-1 cells after co-transfection with Wt or Mt. LASP1 3′-UTR reporter genes (2 μg) and miR-203a-3p mimic or miR-Ctrl (50 nM) d. Each experiment was independently repeated at least three times. Data are presented as mean ± S.D.; * P < 0.05; ** P < 0.01 compared with the miR-Ctrl group, Student’s t-test