Fig. 6

LASP1 is involved in miR-203a-3p mediated tumor suppression. CNE-2 and SUNE-1 cells were co-transfected with either miR-203a-3p mimic or negative control (miR-203a or miR-Ctrl) and either the pSin-EF2-puro-LASP1 (LASP1) plasmid or empty pSin-EF2-puro-Vector (Vector). a Western blotting analysis of LASP1 protein expression. b-f Overexpression of LASP1 abrogated the inhibition on cell proliferation, migration and invasion by miR-203a-3p. Representative results of the MTT assay (b), colony formation assay (c), wounding healing assay (d), transwell migration assay (e) and invasion assay (f). Data are presented as mean ± S.D.; * P < 0.05, ** P < 0.01, one way ANOVA. Each experiment was independently repeated at least three times