Fig. 1
From: β-catenin-mediated YAP signaling promotes human glioma growth
Knockdown of YAP inhibits glioma cell proliferation in vitro. a qPCR analysis for YAP mRNA levels in glioma (n = 18) and nontumor brain tissues (n = 6). b Representative immunoblot of the total extracts from human gliomas (nine samples shown) and nontumor tissues (five samples shown). c Quantification of YAP protein levels in glioma (n = 26) and normal tissues (n = 13) from the immunoblots. d & e Representative images of EdU assay after infecting cells with indicated lentivirus (d) and quantification results (e). The cell proliferation was examined after plating for 48 h. Scale bar, 100 μm. f & g The effect of YAP knockdown on cell proliferation was detected by CCK-8 assay at the indicated time. h & i Representative images (h) and quantitative analysis (i) of U251 colonies infected with indicated lentivirus. Colonies stained with crystal violet 14 days after seeding. * P < 0.05; ** P < 0.01; *** P < 0.001