Fig. 3

YAP modulates the protein level of β-catenin via regulating the activity of GSK3β in destruction complex. a Representative immunoblot of β-catenin in YAP down-regulation or over-expression cells. b-d β-catenin mRNA level in YAP down-regulation or over-expression cells was analyzed by qPCR. e Representative immunoblot of GSK-3β, p-GSK-3β, β-catenin and active-β-catenin after YAP down-regulation. f & g Quantification result of (e). (h) Representative immunoblot of GSK-3β, p-GSK-3β, β-catenin, active-β-catenin and p-β-catenin after YAP over-expression. i Quantification result of (h). * P < 0.05, ** P < 0.01