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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: The oncogenic Golgi phosphoprotein 3 like overexpression is associated with cisplatin resistance in ovarian carcinoma and activating the NF-κB signaling pathway

Fig. 4

GOLPH3L up-regulation activates the NF-κB signaling pathway in ovarian cancer. a Enrichment of transcripts which were up- or downregulated (P < 0.05) by GOLPH3L was determined by their Gene Ontology (GO) terms. b GSEA plot, indicating a significant correlation between the mRNA levels of GOLPH3L expression in ovarian cancer and the NF-κB-activated gene signatures in published datasets. c Analysis of luciferase reporter activity in the indicated cells after transfection with 100 ng pNF-κB–luc plasmids or control-luciferase plasmid. d Western blotting analysis of the expression levels of nuclear p65 proteins in the indicated cells. -tubulin was used as a loading control. e Western blotting analysis of the expression levels of indicate proteins in the indicated cells. a-tubulin was used as a loading control. f Real-time PCR analysis demonstrating an apparent overlap between NF-κB–dependent gene expression and GOLPH3L–regulated gene expression. The pseudo color represents an intensity scale for GOLPH3L versus vector or GOLPH3L siRNA versus control siRNA, calculated by log2 transformation, * P < 0.05

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