Fig. 5

NF-κB signaling pathway is required for GOLPH3L-induced chemoresistance. a Quantification of colony numbers in ovarian cancer cells transfected with vector, IκBα-mut or treated with the NF-κB inhibitor, as determined by colony formation. b Quantification of cisplatin-induced (20 μM) TUNEL-positive cells in ovarian cancer cells transfected with vector, IκBα-mut or treated with the NF-κB inhibitor. Each bar represents the mean ± SD of three independent experiments. c Annexin V-FITC and PI staining of the indicated cells transfected with the vector, IκBα-mut or treated with the NF-κB inhibitor (JSH-23). d-e The luminescence of the intraperitoneal tumor xenografts from different treatment groups at the indicated weeks. f IHC staining demonstrated the expression of TUNEL-positive cells as well as active caspase3 in the indicated tissues, P < 0.05