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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: IL-27 mediates HLA class I up-regulation, which can be inhibited by the IL-6 pathway, in HLA-deficient Small Cell Lung Cancer cells

Fig. 3

IL-27 mediates STAT1 and STAT3 phosphorylation in responsive SCLC cell lines. a Western blot analysis of tyrosine phosphorylated (P)-STAT1, P-STAT3 and total STAT3 proteins in SCLC cells cultured for 20 min with medium (CTR), IL-27 or IFN-γ. Total STAT3 and α-tubulin served as loading controls. No phosphorylation is detected in the IL-27-unresponsive NCI-H146 cells. b Analysis of P-STAT1, STAT1, P-STAT3 and STAT3 proteins in the IL-27-resistant cell line NCI-H146 cultured with medium alone (CTR), IL-27 or IFN-γ for 10 or 30 min. STAT1 phosphorylation was detected only following IFN-γ treatment, while phosphorylation of STAT3 was never detectable. Data are representative of two independent experiments with consistent findings

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