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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: IL-27 mediates HLA class I up-regulation, which can be inhibited by the IL-6 pathway, in HLA-deficient Small Cell Lung Cancer cells

Fig. 5

Comparative analysis of STAT1 and STAT3 phosphorylation in SCLC cell lines stimulated with different cytokines. a Western blot analysis of tyrosine phosphorylated (P)-STAT1 and P-STAT3 proteins in NCI-N592 and NCI-H69 SCLC cells cultured for 30 min with medium (CTR), sIL-6R/IL-6 (hy-IL-6), IL-6, or IL-27. Total STAT3 or STAT1 proteins and α-tubulin served as loading controls. b Kinetics (10 or 30 min) of STAT1 and STAT3 phosphorylation in the IL-27-sensitive cell line NCI-H446 cells cultured with medium alone (CTR), sIL-6R/IL-6 (hy-IL-6), IL-6, IL-27 or IFN-γ. c) Comparison of STAT1 and STAT3 phosphorylation in the IL-27-sensitive cell line NCI-N592 and in the IL-27-non-responsive NCI-H146 cells stimulated with medium alone (CTR), sIL-6R/IL-6 (hy-IL-6), IL-6, and IL-27

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