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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: IL-27 mediates HLA class I up-regulation, which can be inhibited by the IL-6 pathway, in HLA-deficient Small Cell Lung Cancer cells

Fig. 6

Immunofluorescence analysis of sIL-6R/IL-6 and IL-27 effects on HLA class I cell surface expression. a Cytofluorimetric analysis of membrane HLA class I expression in three SCLC cell lines, cultured in the presence of medium (baseline), IL-27 or sIL-6R/IL-6 (hy-IL-6), for 48 h. Isotype-matched Ig control is also shown (ctrIg). Different from IL-27, the GP130 engager sIL-6R/IL-6 does not up-regulate surface HLA class I expression in NCI-H69, NCI-N592 and NCI-H446 cell lines. b Analysis of HLA class I cell surface expression in NCI-H69 cells cultured for 48 h in the presence of medium (baseline), IL-27 (dotted line) or IL-27 plus sIL-6R/IL-6 (hy-IL-6), IL-6 or anti-GP130 mAb (agp130) (black line). sIL-6R/IL-6 and anti-GP130 negatively interfere with IL-27-mediated HLA class I antigen up-regulation, whereas IL-6 is ineffective

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