Fig. 3
Knockdown of Par3 inhibits PCa metastasis in vivo. a Strategy for evaluating the effect of Par3 knockdown on metastasis in vivo. b Representative PET-CT images from orthotopic implantation mouse models by inoculation of PC3-con cells (#1-#3, n = 3) or PC3-shPar3 cells (#4-#6, n = 3). White arrow: bladder, black arrow: orthotopic tumor, dark red arrow: invasion in peritoneal lymph nodes, yellow arrow and field in black broken line: metastatic nodes in liver, bright green: heart. c Orthotopic grafts and liver metastasis are dramatically regressed in PC3-shPar3 inoculated mouse (#4-#6) than control (#1-#3). White arrow: bladder, black arrow: orthotopic tumor, yellow arrow and field in black broken line: metastatic nodes in liver. d H&E staining of liver tissues from orthotopic PC3-shPar3 or PC3-con cells inoculated mice. Field in black broken line and frame: metastatic nodes. Scale Bar: 100 μm for upper panels; 50 μm for lower panels. e IF staining of liver tissues for Par3 expression from orthotopic PC3-shPar3 or PC3-con cells inoculated mice. H-nuclei: human nuclei. Scale Bar: 20 μm