Skip to main content
Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Elevated expression of Par3 promotes prostate cancer metastasis by forming a Par3/aPKC/KIBRA complex and inactivating the hippo pathway

Fig. 4

Knockdown of Par3 decreases the nuclear translocation of the oncogene YAP. a Knockdown of Par3 in PC3 cells enhances the phosphorylation of LATS and YAP so to suppress nuclear translocation of YAP. GAPDH: internal control for cytoplasmic proteins; TBP: internal control for nuclear proteins. b IF staining of nuclear translocation of YAP in PC3-con and PC3-shPar3 cells respectively in vitro. c Nuclear translocation of YAP is impaired in PC3-shPar3 vs. PC3-con cells inoculated orthotopic grafts respectively in vivo. Typical cells are indicated by yellow arrows. d YAP expression in liver tissues from orthotopic PC3-shPar3 or PC3-con cells inoculated mice. H-nuclei: human nuclei. e Expression of YAP in clinical samples by IHC staining. Typical cells are indicated by arrows. Scale Bar: 20 μm

Back to article page