Fig. 6

Knockdown of Par3 decreases the expression of pro-metastatic genes in PC3 cells. a-e Binding sites of TEAD transcription factors (mainly refer to TEAD1, 2 and 4) in the promoter region of MMP1 (a), MMP9 (b), Zeb1 (c), Snail1 (d) and Twist1 (e) respectively. The relative enrichment is significantly improved using either TEAD antibodies or a YAP antibody. A black triangle indicates binding site(s) for TEAD transcription factors. f Expression of MMP1/9, Zeb1, Snail1 and Twist1 is inhibited after Par3 knockdown at both mRNA and protein levels. All data are represented as mean ± SD from triplicate experiments. *: p < 0.05, **: p < 0.01