Fig. 4

PAK5 activates the nuclear translocation of the p65. a Western blot of intracellular p65 from BT549 and MDA-MB-231 cells transfected with the pcDNA3.1-Myc-PAK5 plasmid or vector control. b Western blot of intracellular p65 from BC cells transfected with the PAK5 siRNA or control siRNA. c, d Western blot determined cellular distribution of p65 in BT549 and MDA-MB-231 cells transfected with the pcDNA3.1-Myc-PAK5 plasmid or vector control. e, f Western blot determined cellular distribution of p65 in BC cells transfected with PAK5 siRNA or control siRNA. g, h Immunofluorescent staining of MDA-MB-231 and BT549 cells analyzed by confocal microscopy (magnification, × 400). The antigenic sites of p65 were detected using Alexa Fluor 488 conjugated goat anti-mouse IgG (green). Data are shown as mean ± SD for three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001