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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: The α-melanocyte stimulating hormone/peroxisome proliferator activated receptor-γ pathway down-regulates proliferation in melanoma cell lines

Fig. 4

Analysis of PPARγ translocation into the nucleus and activity in response to 3 M3 exposure. (a, b, c) Immunofluorescence analysis of PPARγ localization in untreated cells (A-C, G-I) and in cells treated with 15 μM 3 M3 for 3 h (D-F, J-L). Immunolabeling with anti-PPARγ antibody and nuclear staining with DAPI. Scale bar: 20 μM. (c) Quantitative analysis of the PPARγ/DAPI colocalization signal in the nucleus. Results are express as fold increase of colocalization signal with respect to the values obtained in untreated cells and are reported as mean value ± SD (%) (*p < 0.01). (d) Transcriptional activity of PPARγ (fold change) by luciferase activity assay in B16-F10 and Mel 13. Cells were transfected with pGL3-(Jwt)3TKLuc reporter construct. After 24 h of transfection, cells were treated with 15 μM 3 M3. The measurement of luciferase activity was carried out 24 h after treatment. The variability of transfection was normalized with β-Gal activity. The results were expressed as fold change with respect to untreated cells. Data are mean values ± SD of three independent experiments performed in triplicate. *p < 0.01 (vs untreated cells)

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