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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: YAP transcriptionally regulates COX-2 expression and GCCSysm-4 (G-4), a dual YAP/COX-2 inhibitor, overcomes drug resistance in colorectal cancer

Fig. 1

YAP and COX-2 are overexpressed in CRC cells and associated with Taxol sensitivity. a YAP and COX-2 expression in HCT15, HCT-116, HT-29, COLO205, FHC and IMCE cells. b Summary of YAP and COX-2 expression from three independent experiments. The expression levels of YAP and COX-2 were determined as in A and quantified by densitometry. **P < 0.01 compared with FHC. c Correlation analysis between YAP and COX-2 expression. d Cell viability of IMCE and HCT-116 cells after treatment with Taxol. Cells were cultured in 96-well plates in the presence or absence of increasing concentrations of Taxol (0.1, 0.3, 1 μM) for 48 h. Cell viability was then determined by using the MTT assay. e Apoptosis induced by Taxol in IMCE and HCT-116 cells. Cells were treated with vehicle or Taxol for 48 h, cells (1 × 105) were collected and incubated with Annexin V and PI. The samples were analyzed by flow cytometry. **P < 0.01 compared with IMCE. Data are representative of at least three independent experiments. Error bars represent SD

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