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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: YAP transcriptionally regulates COX-2 expression and GCCSysm-4 (G-4), a dual YAP/COX-2 inhibitor, overcomes drug resistance in colorectal cancer

Fig. 2

YAP up-regulates COX-2 Expression in both normal and malignant CRC Cells. a IMCE cells were transfected with CMV2-YAP plasmid. Immunoblotting using antibodies against YAP, COX-2 were performed. **p < 0.01,*p < 0.05 compared with control. b Immunoblotting of YAP, COX-2 was performed in HCT15 cells with knockdown of YAP shRNA. **p < 0.01 compared with control. c Immunoblotting of YAP and COX-2 in SW480 cells that were transfected with CMV2-YAP and YAP shRNA successively. **p < 0.01,compared with control; ## p < 0.01compared with YAP. d YAP and COX-2 was detected by western blot in HEK293T cells transfected with YAP expression vectors. **p < 0.01 compared with control. e YAP and COX-2 was detected by western blot in IMCE cells transfected with LATS shRNA. **p < 0.01,*p < 0.05 compared with control;## p < 0.01compared with Scramble. f Immunofluorescent staining of YAP and COX-2 in HCT15 cells that were transfected with YAP shRNA. g Co-IP analysis of interaction of YAP and COX-2. Data are representative of at least three independent experiments. **p < 0.01 compared with control

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