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Table 3 ISH and MLPA results in High Amplified/Non Amplified breast cancers in the internal and external validation set

From: Breast carcinomas with low amplified/equivocal HER2 by Ish: potential supporting role of multiplex ligation-dependent probe amplification

Ā 

DDISH (NĀ =Ā 116)a

Ā 

FISH (NĀ =Ā 98)b

Ā 
Ā 

gene s/n

gene s/n

Ā 

<4.0

ā‰„6.0

<4.0

ā‰„6.0

Ā 

61

55

79

19

MLPA

ā€ƒNA

59 (97%)

11 (20%)

62 (79%)

2 (10.5%)

ā€ƒGAIN

0 (0%)

3 (5%)

7 (9%)

2 (10.5%)

ā€ƒA

2 (3%)

41 (75%)

10 (12%)

15 (79%)

  1. NA Non Amplified, A Amplified, NAĀ <Ā 1.3 mean value, GAINĀ ā‰„Ā 1.3Ā <Ā 1.5 mean value, AĀ ā‰„Ā 1.5 mean value
  2. aInternal set bExternal set