Fig. 3
From: Cooperative antitumor activities of carnosic acid and Trastuzumab in ERBB2+ breast cancer cells
CA and Tz cooperatively arrest cell cycle in G0/G1 and down-regulate ERBB2 levels and signaling in SKBR-3 and BT474 cells. a High resolution DNA flow cytometric analysis of DAPI-stained nuclei of cells cultured for 48 h with control medium (Ctr), CA, Tz or CA + Tz supplemented medium. Both floating and adherent cells were collected for the analysis. Mean values and standard deviation (indicated as vertical bars) from four independent replicates (n = 4) are shown. P < 0.05 (*), P < 0.01 (**), P < 0.001 (***). b Cells were cultured for 7d with control medium (Ctr), CA, Tz or CA + Tz supplemented medium, which was changed every other day. A representative immunoblot analysis is shown, which was performed with anti-ERBB2, anti phospho-Ser473 AKT, anti AKT, anti-Tubulin and anti-Vinculin antibodies on whole cell lysates. Tubulin is shown as loading control for ERBB2. Vinculin is shown as loading control for pAKT and AKT. Numbers on each lane represent ERBB2 protein levels and pAKT/AKT level ratios determined as described in Methods