Fig. 5
From: Cooperative antitumor activities of carnosic acid and Trastuzumab in ERBB2+ breast cancer cells
CA upregulates p62 in SKBR-3 and BT474 cells. Cells were cultured for 7d with control medium (Ctr), CA, Tz or CAÂ +Â Tz supplemented medium, which was changed every other day. A representative immunoblot analysis is shown, which was performed with antibodies specific for p62, ATG5, LC3 and Vinculin on whole cell lysates. Vinculin is shown as loading controls. Numbers on each lane represent p62 and ATG5 levels and LC3 II/I level ratios, determined as described in Methods