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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Cooperative antitumor activities of carnosic acid and Trastuzumab in ERBB2+ breast cancer cells

Fig. 7

CA and CA + Tz dramatically affect lysosomal morphology in SKBR-3 cells. Representative confocal images of SKBR-3 cells cultured for 7d with CA, Tz and CA + Tz and untreated cells. Cells were fixed, permeabilized and challenged with anti-LAMP1 and anti-LAMP2 antibodies to detect lysosomes and Alexa488-conjugated anti-mouse secondary antibody (green signal). Nuclei were stained with DAPI (blue signal). Arrows indicates representative lysosomes in each condition. Of note, in CA and CA + Tz treated cells lysosomes (LAMP1 and LAMP2 positive) appear larger and clustered with respect to untreated cells (arrows). Bar = 20 μm. Insets represent a detail of the original image

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