Fig. 6

SHCBP1 participates in TGF-β1-induced EMT. a Western blot of SHCBP1, vimentin and E-cadherin in the indicated cells in response to treatment with 10 ng/mL TGF-β1 for 0, 24, and 48 h. b Twenty-four hours post-transfection of LV-Control or LV-shSHCBP1 lentivirus, the cells were treated with TGF-β1 (2 ng/ml) for an additional 48 h. The expressions of SHCBP1, E-cadherin and vimentin were detected by Western blot. c Representative images and data of a transwell invasion assay for HS-SY-II and SW982 cells. TGF-β1 stimulation significantly increased the invasiveness of both tumor cell lines compared with that in the absence of TGF-β1 (LV-Control) or LV-shSHCBP1 lentivirus cells. Each bar represents the mean ± SD, **P < 0.01. All images are representative of three independent experiments with similar findings