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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Musashi-2, a novel oncoprotein promoting cervical cancer cell growth and invasion, is negatively regulated by p53-induced miR-143 and miR-107 activation

Fig. 3

MSI-2 regulates CC cell invasion and growth through translational control of c-FOS. a, Western blotting analysis of c-FOS and PTEN in indicated cells. GAPDH served as the loading control. b, qRT-PCR analysis of c-FOS expression in indicated cells. c, Predicted MSI-2 binding site in the 3′-UTR of c-FOS mRNA. d, RNA-immunoprecipitation in CC cells using an anti-IgG control or anti-MSI-2 antibody. c-FOS or GAPDH mRNA abundance in immune-precipitated fraction was determined by qRT-PCR. Results are presented relative to IgG immunoprecipitation, set as 1. e, The c-FOS 3′-UTR reporter constructs containing the wild-type (WT) or mutant (MU) MSI-2 binding sequences were co-transfected with control siRNA or MSI-2 siRNA into CC cells and luciferase activity levels measured. ns, not significant. f, qRT-PCR analysis of c-FOS mRNA in HeLa cells transfected as indicated. g, h and i, Quantification of invasion (g), proliferation (h) and sphere formation (i) of HeLa cells transfected as indicated. * P < 0.05

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