Fig. 6

Upregulation of miR-143 and miR-107 by Mithramycin A via p53 activation decreases MSI-2 levels and suppresses invasion and proliferation of CC cells. a, Western blotting analysis of p53, p21 and MSI-2 in CC cells treated with Mithramycin A (MM). b, miR-143 and miR-107 expression in CC cells treated with Mithramycin A. c, Western blotting analysis of p53 and MSI-2 in CC cells transfected with control or p53 expression vector. d, qRT-PCR analysis of miR-143 and miR-107 in CC cells transfected with control or p53 expression vector. e, Representative images of transwell invasion assays and sphere formation assays (upper panel). Quantification of invasion, proliferation and sphere formation of CC cells treated with Mithramycin A or DMSO (lower panel). ^* P < 0.05