Fig. 4

TAGLN2 knockdown inhibits mesenchymal transition and reduces formation of invadopodia in glioma cells. a Western blot for protein levels of EMT components in lysates (20 μg) from U87MG and U251 cells transfected with siRNA against TAGLN2 and controls. GAPDH was used as a loading control. b Immunofluorescence images of N-cadherin (red), E-cadherin (green), and nuclear (blue) staining in U87MG and U251 at 48 h after siRNA transfection. c Immunofluorescence images for F-actin (red) and nuclear (blue) staining in U87MG and U251 at 48 h after siRNA transfection. Scale bar = 30 μm. d Invadopodia formation was quantified as a percentage of the cells with invadopodia on 30 randomly selected cells in each group. Results are representative of three independent experiments. NT: no treatment; Control: nonsilencing siRNA; si-TAGLN2: siRNAs targeting TAGLN2