Fig. 5

TAGLN2 knockdown inhibits cell proliferation and induces cell cycle arrest. U87MG and U251 cells transfected with TAGLN2 siRNA or controls and characterized in the following assays: (a, b) EdU performed 48 h after transfection (scale bar = 100 μm); (c) growth curve based on OD450 using the CCK-8 assay; (d) cell cycle profiles determined from PI staining in flow cytometry; (e) Western blot to detect expression levels of the known cell cycle regulatory factors indicated. GAPDH was used as a loading control; (f, g) % apoptosis as determined with Annexin V-FITC antibody and PI staining in flow cytometry. Data are shown as the mean ± SEM from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001, relative to control. NT: no treatment; Control: non-silencing siRNA; si-TAGLN2: siRNAs targeting TAGLN2