Fig. 3

RBM38 directly bound to PTEN transcript and regulated its mRNA stability. a-h RBM38 associated with PTEN transcript in vivo. (a-d) BT474 and (e-h) MDA-MB-453 cell lysates were immunoprecipitated with RBM38 and control IgG antibodies followed by RT-PCR (a, e) and qRT-PCR (b-d, f-h), measuring transcript levels of PTEN, HuR, p21 and β-actin within RBM38 or IgG immunocompleses. i-l The half-life of PTEN transcript was up-regulated by RBM38. a, b PTEN mRNA expression in BT474 and MDA-31 MB-453 transfected with RBM38 overexpression lentivirus and the control (NC) after treatment with 5 μg/ml actinomycin D for 0 h, 2 h, 4 h, 6 h and 8 h. c, d PTEN mRNA expression in BT474 and MDA-MB-453 transfected with RBM38 knockdown lentivirus (sh1) and the negative control (SCR) after treatment with 5 μg/ml actinomycin D for 0 h, 2 h, 4 h, 6 h and 8 h. Data were means of three separate experiments and presented as mean ±

SEM. * P < 0.05.