Fig. 5

Reduction of PTEN expression suppressed the cell proliferation driven by RBM38. The effect of PTEN on RBM38-transfected cells was examined by colony formation assay. a-d BT474 and MDA-MB-453 cells transfected with RBM38 overexpression and the control (NC) lentivirus were transduced with siRNA against PTEN. The RBM38-induced growth suppression was decreased after reduction of PTEN in BT474 (a, b) and MDA-MB-453 (c, d) cells. e-h The cells transfected with RBM38 overexpression and the control (NC) lentivirus were treated with the PTEN inhibitors, SF1670 and VO-Ophic, followed by colony formation assay. The RBM38-mediated growth suppression was reduced after treatment with PTEN inhibitors in BT474 (e, f) and MDA-MB-453 (g, h) cells. The fold change was calculated as a ratio of the number of colonies induced by the control (NC) divided by that induced by the RBM38. Data were means 61 of three separate experiments and presented as mean ± SEM. * P < 0.05