Fig. 5

MiR-31 regulates EMT in ESCC cells. a Morphologies of ESCC cell lines after changing the expression of miR-31. b-c QRT-PCR and Western blotting were used to analysis the mRNA and protein levels of EMT markers in ESCC cells after transfection with anti-miR-31. d Immunofluorescence assay was executed the expression of EMT protein markers transfection with anti-miR-31 in ESCC cells. *: P < 0.05; **: P < 0.01