Fig. 9

MiR-31/LATS2/TAZ interaction regulates tumor growth in vivo. a-b Effect of miR-31 on tumor growth in nude minces. Growth curves of tumors resulted from injection of Eca109 cells transfected with anti-miR-31 in nude mice. Immunostaining of LATS2 and TAZ protein stained sections of the transplanted tumors as indicated (original magnification, ×400). c-e Effect of different LATS2 expression on tumor growth in nude mice. Xenograft assay with TE1 stable cells revealed that inhibition of LATS2 improved the volume of the xenograft tumors, while restoration of LATS2 showed a significantly depressed tumor volume. f-g Loss of LATS2 significantly reversed the suppression of tumor growth induced by anti-miR-31. Immunostaining of LATS2 and TAZ protein stained sections was indicated (original magnification, ×400). *: P < 0.05; **: P < 0.01