Fig. 2From: STAT3-blocked whole-cell hepatoma vaccine induces cellular and humoral immune response against HCCThe HCC-vaccine activated the immune system in vivo. BALB/c mice were immunized with the H22 tumor vaccine once a week for three weeks. At the first week after the last immunization, PBMCs were isolated and analyzed by flow cytometry. a The proportion of CD3+DX5− or CD3−DX5+ cells in PBMC of BALB/c were assayed. b & c The expression of CD69 on T cells and NK cells of BALB/c mice are shown (shaded histograms are isotype controls). d & e The proportion of CD11c+ DC cells and the expression of costimulatory molecules CD80 and CD86 on DCs were detected by FACS. PBS, mice immunized with PBS; Lipo, Scrm, and Decoy, mice immunized with Lipo, scrambled, or Decoy-ODN-treated HCC vaccine. Data are expressed as the mean ± SD, statistical significance was determined as *p < 0.05; **p < 0.01 and ***p < 0.001 (n ≥ 4)Back to article page