Fig. 4
From: STAT3-blocked whole-cell hepatoma vaccine induces cellular and humoral immune response against HCC
The HCC vaccine induced a secondary immune response to HCC. BALB/c mice were inoculated subcutaneously with 2 × 106 tumor cells after three rounds of immunization with the HCC-vaccine, and then the PBMCs or tumor infiltrating lymphocytes of BALB/c mice were isolated when the tumor was visible. a The proportion of CD3+DX5− or CD3−DX5+ cells in the PBMCs of BALB/c. b & c The expression of CD69 and CD107a on T cells or NK cells in the PBMCs of BALB/c were analyzed by flow cytometry. d The proportion of Tregs (CD3+CD4+CD25+) in the PBMCs was detected by flow cytometry. e & f The proportion and the expression of CD69 on T cells and NK cells in tumor tissues from BALB/c mice were analyzed by flow cytometry. Data are expressed as the mean ± SD, statistical significance was determined as *p < 0.05 and **p < 0.01 (n = 6)