Fig. 4

Regulation of GPR30 signaling by WDR7-7 to inhibit proliferation in breast cancer cells. The effects of WDR7-7 on GPR30 signaling were detected by (a) Western blotting in MCF-7, T47D, SKBR3, and MDA-MB-468 cells. The knock-down or overexpression of WDR7-7 regulated (b) GPR30 protein expression levels and the phosphorylation of (c) SRC, (d) EGFR, (e) ERK1/2, and (f) Akt. The expression level of GPR30 was normalized to β-actin as the internal control, and the phosphorylation levels SRC, EGFR, ERK1/2, and Akt used the corresponding total protein as the internal control in the same sample. (g) The expression levels of GPR30 mRNA after the overexpression or knock-down of WDR7-7 were determined using qRT-PCR. h The knock-down or overexpression of WDR7-7 regulated cell proliferation, as evidenced by the CCK-8 assay results. Representative data from three independent experiments are shown. *p < 0.05 vs. WDR7-7 control