Fig. 5

Calycosin inhibits cell proliferation by regulating WDR7-7-GPR30 signaling. MCF-7, T47D, SKBR3, and MDA-MB-468 cells were treated for 48 h with 16 μM calycosin, 16 μM calycosin and pCDNA3.1-WDR7-7, or 16 μM calycosin and WDR7-7 shRNA. a-f Western blotting was used to determine the protein expression levels of GPR30, p-SRC, p-EGFR, p-ERK1/2, and p-Akt. The protein expression levels were determined using Western blotting and normalized to either β-actin or the corresponding total protein as the internal control. g The expression levels of WDR7-7 were normalized to those of β-actin. Representative data from three independent experiments are shown. *p < 0.05 vs. control (0 μM); #p < 0.05 vs. 16 μM calycosin alone. h Schematic illustration of the proposed mechanism by which calycosin inhibits proliferation in breast cancer cells. This activity depends on WDR7-7-GPR30 signaling and a feedback loop that involves miR-375, RASD1, and ERα