Fig. 1

Silencing CLDN6 affected chemoresistance in response to ADM in MCF-7/MDR cells. a RT-PCR analysis of CLDN6 expression in MCF-7/MDR and MCF-7 cells. GAPDH was used as a control. b CLDN6, P-gp and GSTP1 expression in MCF-7/MDR and MCF-7 cells by using western blot assay. c P-gp and CLDN6 expression when silencing CLDN6 transiently in MCF-7/MDR cells by using western blot analysis. d P-gp and CLDN6 expression when silencing CLDN6 in screened stable clones in MCF-7/MDR cells by using western blot analysis. e IC₅₀ of ADM, DDP, and 5-FU when RNAi CLDN6 by shRNA in MCF-7/MDR cells. f DAPI nuclear staining was applied to detect ADM-inducing apoptosis in CLDN6-silenced MCF-7/MDR cells (MCF-7/MDR-sh-CLDN6). g Cleaved-PARP and cleaved-caspase-9 were detected in MCF-7/MDR-sh-CLDN6 cells by using western blot. h Expression of γH2AX, p53, and p-p53 in MCF-7/MDR-sh-CLDN6 cells when treated with ADM. *, P < 0.05; **, P < 0.01