Fig. 3

Silencing GSTP1 decreased chemoresistance in response to ADM in CLDN6-overexpressing MCF-7 cells. a GSTP1 expression was detected by western blot when CLDN6 overexpressed. b GST activity assay increased when overexpression CLDN6 in MCF-7 cells. c Three different GSTP1 specific shRNA constructs were utilized to screen the optimal sequence in MCF-7/CLDN6 cells by using western blot. d GST activity assay decreased when silenced GSTP1 expression in MCF-7/CLDN6 cells. e IC₅₀ of ADM, DDP, and 5-FU when inhibited GSTP1 expression in CLDN6-overexpressing MCF-7 cells. f DAPI nuclear staining was applied to detect ADM-inducing apoptosis in MCF-7/CLDN6-sh-GSTP1 cells. g Cleaved-PARP, cleaved-caspase-9 and cleaved-caspase-7 expression in MCF-7/CLDN6-sh-GSTP1 treated with the same dose of ADM by using western blot assay. h Expression of γH2AX, p53, and p-p53 in GSTP1 silenced MCF-7/CLDN6 cells when treated with ADM. i GSTP1 expression was higher in MCF-7/MDR cells than in MCF-7 cells. j Silencing GSTP1 in MCF-7/MDR cells was verified by western blot. k IC₅₀ of ADM and DDP when silenced GSTP1 expression in MCF-7/MDR cells. *,P < 0.05; **, P < 0.01