Fig. 4
From: CLDN6 promotes chemoresistance through GSTP1 in human breast cancer
Overexpression of GSTP1 reversed CLDN6 silence-induced decreasing chemoresistance in MCF-7/MDR cells. a RNAi inhibited CLDN6 downregulated GSTP1 expression in MCF-7/MDR cells. b GST activity assay decreased when silenced CLDN6 in MCF-7/MDR cells. c Western blot verified GSTP1 overexpression in MCF-7/MDR-sh-CLDN6 cells. d IC50 of ADM and DDP when overexpressed GSTP1 in MCF-7/MDR-sh-CLDN6 cells. e DAPI nuclear staining was applied to detect ADM-inducing apoptosis when overexpressed GSTP1 in MCF-7/MDR-sh-CLDN6 cells. f Cleaved-PARP and cleaved-caspase-9 expression in GSTP1-overexpressed MCF-7/MDR-sh-CLDN6 cells treated with the same dose of ADM by using western blot assay. g Expression of γH2AX, p53, and p-p53 in GSTP1 overexpressed MCF-7/MDR-sh-CLDN6 cells when treated with ADM. *, P < 0.05; **, P < 0.01