Skip to main content
Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: RETRACTED ARTICLE: TGF-β2 initiates autophagy via Smad and non-Smad pathway to promote glioma cells’ invasion

Fig. 4

Impairing autophagy inhibits MMP generation and secretion as well as EMT-like process, which lead to deficient glioma invasion. a mRNA expression levels of MMP family genes (MMP1–3, 7–9, 11–17) in U251, T98, and U87 NTC and Baf-treated cells with TGF-β. b Immunoblot analysis showing the effects of Baf on MMP2, MMP9, and MMP14 synthesis in glioma cell lines after exposed to TGF-β2. c The MMP2 and MMP9 secretion levels in supernate obtained from NTC and Baf-treated cells were tested using an ELISA kit. d The expression levels of EMT markers (N-cadherin, E-cadherin, Vimentin, β-catenin, and Slug2) in different groups were tested by Western blots. e, f Immunofluorescences observed N-cadherin and Vimentin expression in glioma cell lines after treated with TGF-β2 and Baf, Representative images of three independent experiments are shown (scale bars, 50 μm). (Student’s t-test; Columns, means of triplicate assays. Bars, SD. **, P < 0.01; ***, P < 0.001)

Back to article page