Fig. 3

MiR-193a modulates apoptosis and suppresses PCa cell migration and invasion in vitro. (a-c) Annexin V-FITC/PI apoptosis assays were applied to show the modulation of apoptosis by EZH2 and miR-193a in PC3 and DU145 cells. (d1-d2, e1-e2, f-g) Transwell assays were used to access the influence of overexpressed miR-193a on migration and invasion ability in PC3 and DU145 cells. (h-j) GSEA showing that negatively enriched expression of gene sets was involved in hallmarks of TGF-β, TNF-α via NF-kB, and KRAS prostate up cell signalings in miR-193a-overexpressing PCa cells. Each bar represents the mean ± SD of three independent experiments. *P < 0.05