Fig. 4

FGFR1 mediated the anti-glycolytic function of miR-361-5p by regulating the activity of PDHK1 and LDHA. a Measurement of FGFR1 mRNA expression in Dicer −/− and +/+ (left panel) or WT (middle panel) MEFs by using qRT-PCR. Detection of FGFR1 protein expression in indicated cells (right panel). b Luciferase reporter assay for FGFR1 WT and Mut 3’UTRs. c ECAR assays for indicated cells. Glucose uptake (d) and lactate production (e) were detected in BC cells transfected with or without miR-361-5p or FGFR1. Glucose uptake (f) and lactate production (g) were detected in BC cell transfected with or without anti-miR-361-5p or siFGFR1. h Immunofluorescence staining of Glut1 in indicated cells. i Western blot analysis was used to detect the phosphorylation and activity of the glycolysis-associated enzymes in MDA-MB-231 cells. j Colony formation assays were used to detect the proliferation of indicated cells. k Transwell assays were used to detect the effect of FGFR1 on invasion ability of BC cells. *P < 0.05, **P < 0.01