Fig. 5

MiR-361-5p suppresses the invasion and metastasis of BC by targeting MMP-1. a Schematic diagram of the luciferase reporter containing the WT or MUT miR-361-5p binding sequence in the 3’UTR of MMP-1 mRNA. b Luciferase reporter assays for MMP-1 WT and MUT 3’UTR. c The expression level of mRNA and protein in BC cells transfected with miR-361-5p or anti-miR-361-5p. d Transwell assays were performed to evaluate the effect of MMP-1 on invasion ability of BC cells transfected with miR-361-5p or anit-miR-361-5p. e Lung metastasis model was used to detect the metastatic ability of BC cells in SCID mice