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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Patient-derived xenograft in zebrafish embryos: a new platform for translational research in gastric cancer

Fig. 3

Proliferation of gastric cell lines in the zebrafish xenografted model under different treatment groups of 5-FU at 2 dpt. 200–300 cells of AGS cell line (a) and SGC-7901 cell line (b) were fluorescently labeled in red and microinjected to the yolk sac of each zebrafish embryo at 48 hpf (0 dpi). After 24 h (0 dpt), zebrafish embryos were microinjected with vehicle control (0.1% DMSO), 6.5 ng/embryo, and 65 ng/embryo of 5-FU respectively for the 2 days. Cell number was determined by sacrificing embryos at 0 dpt and 2 dpt. Cell number at 0 dpt was normalized to 1 and set as baseline. Quantitative values are means ± SEM from 30 independent individuals. Significance at different treatment group was considered when P values were lower than 0.05. (***) indicates statistical significance P < 0.001. hpf: hours post fertilization, dpi: days post injection, dpt: days post treatment

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