Fig. 6

Quantification of patient samples (#6 - #9) engraftment with three chemotherapeutic drugs. Patient samples (#6 and #7) were treated with 5-FU at 6.5 ng/embryo and 65 ng/embryo for 2 days (a). Patient samples (#8 and #9) were treated with apatinib (0.5 μM), docetaxel (5 μM), and 5-FU (65 ng/embryo) for 2 days (b). Cell number at 0 dpt was normalized to 1 and set as baseline. Fold change was determined by sacrificing embryos at 0 dpt and 2 dpt. Quantitative values are means ± SEM from 30 independent individuals. Significance at different treatment group was considered when P values were lower than 0.05. (*) indicates statistical significance P < 0.05 and (***) P < 0.001. dpt: days post treatment