Fig. 3
Apigenin activates p53 leading to an increase of catalase expression. a BC3 and BCBL1 cell lines were treated with apigenin 25μM for 24 h. p53, p21 and catalase expression was assessed by western blotting analysis. β-Actin was used as loading control. Numbers are calculated by quantitative densitometric analysis and indicate the ratio of specific proteins versus β-Actin. Data are representative of at least three independent experiments. b BC3 cells were transiently transfected with si-p53 vector to silence p53 protein for 48 h and treated with apigenin 25 μM for the last 24 h. p53, p21 and catalase expression was evaluated by western blotting. Numbers are calculated by quantitative densitometric analysis and indicate the ratio of specific proteins versus β-Actin. Data are representative of at least three independent experiments. c p53-silenced or control BC3 cell survival was assessed by trypan blue exclusion assay. The histograms represent the mean of the percentage of cell survival plus SD of at least three independent experiments. *p< 0.05. d The expression levels PARP uncleaved and cleaved (ClPARP) were assessed by western blot analysis in p53-silenced or control BC3 cells. β-Actin was used as loading control. Numbers are calculated by quantitative densitometric analysis and indicate the ratio of clPARP versus β-Actin