Fig. 5

Down-regulation of p53 reduces apigenin-mediated STAT3 inhibition that in turn activates p53/p21. a BC3 cells were p53-silenced as described in Fig. 3b. Western blotting analysis was used to assess pSTAT3 (Tyr 705) expression. Numbers are calculated by quantitative densitometric analysis and indicate the ratio of phosphorylated (p-STAT3) versus total protein (STAT3) and total protein (STAT3) versus β-Actin. Data are representative of at least three independent experiments. b BC3 cells were treated with AG490 (50 μM) for 24 h and pSTAT3 (Tyr 705), total STAT3 and p21 expression was investigated by western blot. Numbers are calculated by quantitative densitometric analysis and indicate the ratio of phosphorylated (p-STAT3) versus total protein (STAT3) and total protein (STAT3) or p21 versus β-Actin. Data are representative of at least three independent experiments