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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Hypermethylation and loss of retinoic acid receptor responder 1 expression in human choriocarcinoma

Fig. 1

RARRES1 methylation and expression pattern. a Gene locus of RARRES1 on chromosome 3. The ideogram shows the RARRES1 locus on 3q25.32. The genetic region is drawn schematically with the coding regions as black squares. Two CpG regions within the promoter were selected for further analysis. CpGs are marked with black pins. Red arrows symbolize the sequencing primers for the pyrosequencing reaction. b Heat-map for the promoter methylation pattern. DNA of 3rd trimester placentas (P; from n = 74 individuals), 3rd trimester trophoblasts (Tr; from n = 15 individuals), the trophoblast-like cell line Swan71 (n = 1) and the choriocarcinoma cell lines Jeg-3, JAR and BeWo (n = 1 each) were analyzed using pyrosequencing. The methylation intensity was illustrated by heat map coloring as indicated by the scale below, where green represents the unmethylated state (0%) and black the completely methylated state (100%). c Gene-expression analysis of RARRES1 in placental tissues (from n = 10 individuals), primary trophoblasts (from n = 5 individuals) as well as Swan71 cells and the choriocarcinoma cell lines (n = 9). Gene expression is indicated as relative to RARRES1 expression of 3rd trimester placentas (P). d QRT-PCR analysis of AZA-treated cell lines relative to control (n = 9). Significance is marked by asterisks (* p < 0.05, ** p < 0.005). Data represent mean ± SEM

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