Skip to main content
Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Hypermethylation and loss of retinoic acid receptor responder 1 expression in human choriocarcinoma

Fig. 4

Correlation of cell density and RARRES1 expression. a RARRES1 expression was analyzed in human third trimester placental sections by immunofluorescence staining. RARRES1 positivity is indicated by green. Nuclei are blue. a (a-b) represent the villous, c the extravillous part. b is a magnification of the square in a. White arrow heads indicate RARRES1 positive VTs, which are in cell-cell contact with the SCT. The bars equal 100 μm. b Scheme of the experimental procedure. Swan71 cells were seeded at different cell densities and RNA was isolated after 48 h. By qRT-PCR we compared the expression pattern of different genes in dependence of low to high cell densities normalized to 18SrRNA. c qRT-PCR analysis of RARRES1 (a and b), RARα, β, γ and RXRα (c) as well as E-Cadherin, N-Cadherin and TJP1 (d) expression in Swan71 (a, c and d) and Jeg-3 (b) cells. Gene expression was normalized to 18 s rRNA expression and set relative to the expression level observed at 0.75x104 cells per ml. Significance is marked by asterisks (* p < 0.05, ** p < 0.005) and calculated relative to the expression at a cell density of 0.75*104/ml. Data represent mean ± SEM

Back to article page